CLONING AND CHARACTERIZATION OF THE MURINE ACTIVIN RECEPTOR-LIKE KINASE-1 (ALK-1) HOMOLOG

Multiple serine/threonine kinases were detected in a bone marrow strom al cell line. One of these, like kinase-1 (ALK-1) homolog was cloned a nd sequenced. The expressed recombinant protein (62 kDa) was consisten t in size with that predicted by the cDNA (58.6 kDa). On Western blots , a goat polyclonal antibody detected the native ALK-1 protein in bone marrow stromal cells, lung, brain, kidney and spleen. Two protein spe cies of 60 kDa and 72-76 kDa were detected. Glycosylation events or al ternative splicing may account for the larger protein specie. (C) 1995 Academic Press, Inc.