INDUCTION OF LIVER CYTOCHROME-P450 2B1 BY BETA-IONONE IN SPRAGUE-DAWLEY RATS

Induction of liver cytochrome P450 2B1 by beta-ionone was investigated in male and female Sprague Dawley rats. Administration of beta-ionone subcutaneously 72 and 48 hr before sacrificing the animals not only s ignificantly induced the liver microsomal activity of pentoxyresorufin O-dealkylase, but also clearly increased in the level of cytochrome P 450 2B1 protein. The induction of cytochrome P450 2B1 by beta-ionone w as much greater in male rats than in female rats. A slot blot analysis showed that the mRNA level was increased from 6 hr after treatment wi th beta-ionone in male rats and from 12 hr after treatment in female r ats. Taken together, the present results indicate for the first time t hat the induction of cytochrome P450 2B1 by beta-ionone might be regul ated by the accumulation of mRNAs. (C) 1995 Academic Press, Inc.