GENES LEAVE THEIR CHARACTERISTIC MARKS ON THE MELTING PROFILE OF PLASMID DNAS

Authors
ZUBRZYCKI IZ KLUMP HH
Citation
Iz. Zubrzycki et Hh. Klump, GENES LEAVE THEIR CHARACTERISTIC MARKS ON THE MELTING PROFILE OF PLASMID DNAS, Zeitschrift fur Naturforschung. C, A journal of biosciences, 50(9-10), 1995, pp. 652-655
Citations number
19
Categorie Soggetti
Biology
Journal title
Zeitschrift fur Naturforschung. C, A journal of biosciences → ACNP
ISSN journal
09395075
Volume
50
Issue
9-10
Year of publication
1995
Pages
652 - 655
Database
ISI
SICI code
0939-5075(1995)50:9-10<652:GLTCMO>2.0.ZU;2-5
Abstract
High resolution melting profiles of four linearised plasmids (pUC9, pG V403, pHP2, and pBR322) were recorded by means of UV absorbance vs. te perature scaning. The set of transitions obtained for each plasmid are compared to each another and to the transitions obtained for their ex cised particular antibiotica resistance gene. It can be shown that eac h gene leaves a charactersitic mark on the melting profile of its pare ntal plasmid.