A REACTIVE MACROMOLECULAR MATRIX FOR PROTEIN IMMOBILIZATION ON A GOLDSURFACE - APPLICATION IN SURFACE-PLASMON RESONANCE

Authors
MILLOT MC MARTIN F BOUSQUET D SEBILLE B LEVY Y
Citation
Mc. Millot et al., A REACTIVE MACROMOLECULAR MATRIX FOR PROTEIN IMMOBILIZATION ON A GOLDSURFACE - APPLICATION IN SURFACE-PLASMON RESONANCE, Sensors and actuators. B, Chemical, 29(1-3), 1995, pp. 268-273
Citations number
13
Categorie Soggetti
Engineering, Eletrical & Electronic","Instument & Instrumentation
Journal title
Sensors and actuators. B, Chemical → ACNP
ISSN journal
09254005
Volume
29
Issue
1-3
Year of publication
1995
Pages
268 - 273
Database
ISI
SICI code
0925-4005(1995)29:1-3<268:ARMMFP>2.0.ZU;2-9
Abstract
A reactive copolymer bearing functions able to react at room temperatu re with amino groups has been immobilized on a cysteamine-coated gold surface. Polymer immobilization, covalent coupling of rabbit immunoglo bulin G and antigen-antibody reactions were studied using a surface pl asmon resonance (SPR) technique. The effect of a protein A layer on th e antigen-antibody reaction was examined.