FIXATION OF DNA DIRECTLY ON OPTICAL WAVE-GUIDE SURFACES FOR MOLECULARPROBE BIOSENSOR DEVELOPMENT

Authors
KARYMOV MA KRUCHININ AA TARANTOV YA BALOVA IA REMISOVA LA VLASOV YG
Citation
Ma. Karymov et al., FIXATION OF DNA DIRECTLY ON OPTICAL WAVE-GUIDE SURFACES FOR MOLECULARPROBE BIOSENSOR DEVELOPMENT, Sensors and actuators. B, Chemical, 29(1-3), 1995, pp. 324-327
Citations number
5
Categorie Soggetti
Engineering, Eletrical & Electronic","Instument & Instrumentation
Journal title
Sensors and actuators. B, Chemical → ACNP
ISSN journal
09254005
Volume
29
Issue
1-3
Year of publication
1995
Pages
324 - 327
Database
ISI
SICI code
0925-4005(1995)29:1-3<324:FODDOO>2.0.ZU;2-L
Abstract
Covalent attachment of DNA molecules to the surface of silicon nitride without an intermediate polymer layer is described. II is shown that the silicon nitride has a sorption capacity no less than 200 pg/mm(2), hybridization efficiency being close to 100%. The expected value of r efractive index changes in the molecular layer under hybridization is estimated as sufficient for the operation of two kinds of waveguide-co ntaining transducers.