HPLC PURIFICATION OF SYNTHETIC OLIGODEOXYRIBONUCLEOTIDES CONTAINING BASE-MODIFIED AND BACKBONE-MODIFIED SEQUENCES

Applications for a new polymer resin, PolyFlo(R), are described for bo th the small-scale and large-scale purification of synthetic oligodeox yribonucleotides varying in length from 18-41 bases. The unique proper ties of this innovative resin provide >95% purified full-length oligod eoxyribonucleotides with greater than 90% yield starting from either c rude trityl-on or trityl-off modified as well as base (biotin)- or bac kbone (e.g., phosphorothioate)-modified products. Full biological acti vity of recovered nucleic acid is retained and the resin is capable of removing contaminating endotoxins during purification. The resin perf ormance is predictable and reliable. The resin can be regenerated easi ly and is particularly economic when employed directly in ammonia or w ith the trityl-off option. PolyFlo meets the requirements of current G ood Manufacturing Practices.