MAREKS-DISEASE VIRUS SEROTYPE-2 GLYCOPROTEIN-I GENE - NUCLEOTIDE-SEQUENCE AND EXPRESSION BY A RECOMBINANT BACULOVIRUS

Citation
Hk. Jang et al., MAREKS-DISEASE VIRUS SEROTYPE-2 GLYCOPROTEIN-I GENE - NUCLEOTIDE-SEQUENCE AND EXPRESSION BY A RECOMBINANT BACULOVIRUS, Journal of veterinary medical science, 58(11), 1996, pp. 1057-1066
Citations number
54
Categorie Soggetti
Veterinary Sciences
ISSN journal
09167250
Volume
58
Issue
11
Year of publication
1996
Pages
1057 - 1066
Database
ISI
SICI code
0916-7250(1996)58:11<1057:MVSGG->2.0.ZU;2-1
Abstract
In the Marek's disease virus (MDV) serotype 2 (MDV2) genome, a gene eq uivalent to the glycoprotein I (gI) of other alphaherpesviruses was id entified and sequenced. The primary translation product comprises 355 amino acids with a M(r) of 38.4 kDa. The predicted amino acid sequence possesses several characteristics typical of membrane glycoproteins, including a N-terminal hydrophobic signal sequence, C-terminal transme mbrane and cytoplasmic domains, and extra-cellular region containing t hree potential N-linked glycosylation sites. Compared to other MDV ser otypes, MDV2 gI showed 49% identity with MDV I gI, and 36% identity wi th HVT gI at the amino acid level. In transcriptional analyses, a 3.5 kb mRNA which starts between 56 and 147 bps upstream of the potential translational initiation codon of gI was identified as the gI-specific transcript. By a recombinant baculovirus, this potential gI encoding region was expressed as two specific products 45 and 43 kDa. Both prod ucts were susceptible to tunicamycin treatment, indicating that they w ere glycoprotein. Further, the expressed gI reacted with all chicken-a ntisera raised to each of the three serotypes of MDV (strains GA, SE-1 , and FC126), suggesting that gI is expressed by all three serotypes o f MDV in infected cells and conserves common antigenic epitope(s) beyo nd serotypes.