J. Perezlesher et Wj. Hickey, USE OF AN S-TRIAZINE NITROGEN-SOURCE TO SELECT FOR AND ISOLATE A RECOMBINANT CHLOROBENZOATE-DEGRADING PSEUDOMONAS, FEMS microbiology letters, 133(1-2), 1995, pp. 47-52
A novel mating system, which uses the s-triazine nitrogen source (cyan
uric acid, CA) as a selective nutritional marker, was developed to eva
luate mobility and facilitate identification of chlorobenzoate (CBA) d
egradation genes in Pseudomonas o aeruginosa strain JB2. Matings were
done between strain JB2, which is unable to grow with CA, and a CA-uti
lizer Pseudomonas sp. strain D on 2-CBA-CA and 3-CBA-CA media. Isolate
s were recovered only from the 2-CBA-CA and were all identified as str
ain D derivatives. Subtractive hybridization analysis of genomic DNAs
from the parental strains and a selected isolate, Pseudomonas sp. stra
in JPL, was used to determine that the latter organism had acquired ap
prox. 16.1 kb of DNA from strain JB2.