Rg. Delmastro et al., HUMAN CHROMOSOME-SPECIFIC CDNA LIBRARIES - NEW TOOLS FOR GENE IDENTIFICATION AND GENOME ANNOTATION, PCR methods and applications, 5(2), 1995, pp. 185-194
To date, only a small percentage of human genes have been cloned and m
apped. To facilitate more rapid gene mapping and disease gene isolatio
n, chromosome 5-specific cDNA libraries have been constructed from Fiv
e sources. DNA sequencing and regional mapping of 205 unique cDNAs ind
icates that 25 are from known chromosome 5 genes and 138 are from new
chromosome 5 genes (a frequency of 79.5%). Sequence complexity estimat
es indicate that each library contains similar to 20% of the similar t
o 5000 genes that are believed to reside on chromosome 5. This study m
ore than doubles the number of genes mapped to chromosome 5 and descri
bes an important new tool for disease gene isolation.