GENOMIC CLONING AND CHROMOSOMAL ASSIGNMENT OF RAT REGUCALCIN GENE

The gene for a Ca2+-binding protein regucalcin was cloned from a rat g enomic library which was constructed in lambda FIX II by screening wit h radiolabeled probe (complementary DNA of rat liver regucalcin). Posi tive clone had 19.9 kb insert of size and contained four exons of the gene coding for a rat regucalcin. These exons included the partial cod ing sequence (61.2% of open reading frame) and the entire 3'-untransla ted region of the gene. The nucleotide sequence of exons completely ag reed with that of a rat regucalcin cDNA clone. The sequence analysis o f the clone showed that the identifier sequence and two simple repeate d sequences exist in the intron of the gene. Moreover, chromosomal loc ation of the rat regucalcin gene was determined by direct R-banding fl uorescence in situ hybridization (FISH) method with the 19.9 kb clone containing four exons. The regucalcin gene was localized on rat chromo some Xq11.1-12 proximal end.