IDENTIFICATION AND QUANTITATIVE-DETERMINATION OF BIOGENIC-AMINES AND THEIR METABOLITES BY GAS-CHROMATOGRAPHY NEGATIVE-ION CHEMICAL-IONIZATION MASS-SPECTROMETRY (GC-NICIMS)

Biogenic amines in me brain tissue of the American Cockroach have been identified and quantified by an extraction derivatisation procedure u sing ditrifluoromethyl benzoyl chloride (DTFMBCL) and isopropyldimethy l silylchloride (IPDMS CL). The molecular ion of these N-DTFMB-XIPMDMS derivatives carried most of the ion current under NICI conditions, wi th potential limit of detection below the picogram level. 5-Hydroxytry ptamine and N-acetyl-5-hydroxytryptamine have been quantified by conve rting their O- and O-, N-acetylates with perfluoroacyl anhydrides. The limit of detection of these spirocyclic products on-column was less t han 15 pg.