DETERMINATION OF URIC-ACID AND XANTHINE HYPOXANTHINE IN BLOOD USING IMMOBILIZED ENZYMES IN FLOW-INJECTION SYSTEM

Authors
HUSSAIN G YAQOOB M NABI A MASOOM M
Citation
G. Hussain et al., DETERMINATION OF URIC-ACID AND XANTHINE HYPOXANTHINE IN BLOOD USING IMMOBILIZED ENZYMES IN FLOW-INJECTION SYSTEM, Journal of the Chemical Society of Pakistan, 17(2), 1995, pp. 109-112
Citations number
10
Categorie Soggetti
Chemistry
Journal title
Journal of the Chemical Society of Pakistan → ACNP
ISSN journal
02535106
Volume
17
Issue
2
Year of publication
1995
Pages
109 - 112
Database
ISI
SICI code
0253-5106(1995)17:2<109:DOUAXH>2.0.ZU;2-G
Abstract
A flow injection system containing on-line immobilized enzyme mini-col umns of urate oxidase and xanthine oxidase/urate oxidase for the deter mination of uric acid and xanthine/hypoxanthine is described. For the determination of xanthine and hypoxanthine, a xanthine oxidase column was incorporated on-line prior to the mate oxidase column. The results of the uric acid analysis in serum were compared with the commercial kit method in use in hospitals with a correlation coefficient of 0.99 and r.s.d. of 1% (n = 6). The method is fast and the cost per analysis is greatly reduced.