ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR DETECTION OF FELINE SERUM AMYLOID A PROTEIN BY USE OF IMMUNOLOGICAL CROSS-REACTIVITY OF POLYCLONAL ANTI-CANINE SERUM AMYLOID A PROTEIN ANTIBODY

Immunological cross-reactivity between feline and canine serum amyloid A protein (SAA) was studied to establish enzyme-linked immunosorbent assay (ELISA) with heterologous antibody. Purified feline SAA formed a precipitin line in immunogel double diffusion with anti-canine SAA an tibody. Immunological cross-reactivity was similarly observed in ELISA . In sandwich ELISA with anti-canine SAA antibody, a dose-response cur ve was obtained over the range of 2 mu g/ml to 123 mu g/ml of purified feline SAA. From the present findings, the sandwich ELISA is found to have high repeatability for quantitation of purified feline SAA and m ay be applicable to determine the serum concentration of feline SAA.