ISOLATION OF VIABLE CELLS IN CANINE TRANSMISSIBLE SARCOMA (CTS) USINGDENSITY GRADIENT CENTRIFUGATION

Authors
OKAMURA Y MINAMI S MATSUHASHI A FUJINAGA T
Citation
Y. Okamura et al., ISOLATION OF VIABLE CELLS IN CANINE TRANSMISSIBLE SARCOMA (CTS) USINGDENSITY GRADIENT CENTRIFUGATION, Journal of veterinary medical science, 58(11), 1996, pp. 1149-1151
Citations number
18
Categorie Soggetti
Veterinary Sciences
Journal title
Journal of veterinary medical science → ACNP
ISSN journal
09167250
Volume
58
Issue
11
Year of publication
1996
Pages
1149 - 1151
Database
ISI
SICI code
0916-7250(1996)58:11<1149:IOVCIC>2.0.ZU;2-V
Abstract
Density gradient centrifugation using Ficoll-Conray solution was attem pted to isolate viable canine transmissible sarcoma (CTS) cells. The v iability of isolated tumor cells increased from about 50% to >90%, and the yield of CTS cells was >50% with over 99% purity, in when an isol ation solution density of 1.05 was used.