CHARACTERIZATION OF A FERROCENE CONTAINING POLYACRYLAMIDE-BASED REDOXGEL FOR BIOSENSOR USE

A novel neutral ferrocene-containing polyacrylamide-based redox gel wa s prepared from the copolymerization of vinylferrocene (VF) with acryl amide and N,N'-methylenebisacrylamide in a one-step procedure, Hydroxy propyl-beta-cyclodextrin (HPCD) was used to convert water-insoluble VF into the water-soluble VF-HPCD inclusion complex, allowing the copoly merization to be performed in aqueous solution. A new ternary catalyst , consisting of flavin mononucleotide, H2O2, and N,N,N',N'-tetramethyl ethylenediamine, was developed for initiating the polymerization, A re dox gel electrode, fabricated by covering the redox gel on the surface of a carbon paste electrode with a dialysis membrane, exhibits quasir eversible electrochemical characteristics. The electrocatalytic proper ties of a glucose biosensor prepared by entrapping glucose oxidase in the redox gel were investigated. The simplicity and reproducibility of its preparation make this new redox gel a good candidate for biosenso r applications.