A. Zhiri et al., TISSUE-CULTURES OF TAXUS-BACCATA AS A SOURCE OF 10-DEACETYLBACCATIN-III, A PRECURSOR FOR THE HEMISYNTHESIS OF TAXOL, Biological chemistry Hoppe-Seyler, 376(10), 1995, pp. 583-586
Callus cultures of Taxus baccata L. cv. stricta were induced from hypo
cotyl and leaf explants on Woody Plant medium (hormone-free or additio
nated with phytohormones). Under continuous dark condition, adventitio
us roots were regenerated from hypocotyl- and leaf-derived callus cult
ures. Antibodies raised in rabbits against 10-succinyl-10-deacetylbacc
atin III were used for the detection and the semi-quantitative determi
nation of 10-deacetylbaccatin III in Taxus cultures. The presence of 1
0-deacetylbaccatin III in callus extracts was confirmed by TLC, HPLC u
sing a photodiode array detector and mass spectrometry (CI-MS). The hi
ghest equivalent content of the taxoid derivatives (7.83 mg/100 g dry
wt.) was detected in an extract from leaf-derived callus.