C. Brauer et al., THE GENE FOR THE MAJOR PROTEIN (SVSP109) OF BOVINE SEMEN - STRUCTURE AND PROMOTER ANALYSIS, Biological chemistry Hoppe-Seyler, 376(10), 1995, pp. 631-636
We have characterized the gene of SVSP109, specifying the bovine secre
tory protein SVSP109, which is synthesized in a tissue- as well as spe
cies-specific manner. Approximately 1.3 kb upstream of the SVSP109 gen
e, the 3'-end of another gene designated HG5 was identified. The HG5 g
ene fragment comprises exon (n-1) and exon (n), separated by an intron
. Exon (n) contains the complete 3'-untranslated region, whereas exon
(n-1) encodes the C-terminal part of a hitherto unknown protein with h
igh homology to SVSP109. The sequenced region of 6956 bp of a bovine g
enomic clone comprised the complete SVSP109 gene, which is made up of
five exons and four introns. Primer extension analysis and RTPCR of po
ly(A+)RNA from seminal vesicle revealed that the first exon 1 extends
to a position 34 bp downstream of the TATA sequence. Employing a CAT a
ssay, a definitive but weak promoter activity was detected in pCATeSVS
P15 (base pairs -639 to +574) and pCATeSVSP10 (base pairs -639 to +198
); pCATeSVSP6 (base pairs -262 to +65) displayed promoter activity sim
ilar to the positive control. We conclude from these results that the
TATA sequence located at position -34 is part of the functional promot
er of the SVSP109 gene.