CHROMOSOME SORTING OF PRIMATES - ISOLATION AND IDENTIFICATION OF Y-CHROMOSOME IN GREEN MONKEYS

Single laser flow cytometry was applied to the karyotype analysis of g reen monkeys. Clear sex difference in flow karyotype was recognized in this monkey, because Y chromosome could be identified as a single pea k in the histogram of male specimens. We could isolate Y chromosome of this species by the use of a cell sorter, and demonstrate by polymera se chain reaction that the sorted-out chromosomes contained the Y chro mosome specific nucleotide sequence (SRY). This chromosome sorting tec hnique provides a powerful strategy for constructing the DNA library s pecific to Y chromosome in this species.