CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF FRUCTOSE 6-PHOSPHATE, 2-KINASE-FRUCTOSE 2,6-BISPHOSPHATASE

Authors
ISTVAN ES HASEMANN CA KURUMBAIL RG UYEDA K DEISENHOFER J
Citation
Es. Istvan et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF FRUCTOSE 6-PHOSPHATE, 2-KINASE-FRUCTOSE 2,6-BISPHOSPHATASE, Protein science, 4(11), 1995, pp. 2439-2441
Citations number
21
Categorie Soggetti
Biology
Journal title
Protein science → ACNP
ISSN journal
09618368
Volume
4
Issue
11
Year of publication
1995
Pages
2439 - 2441
Database
ISI
SICI code
0961-8368(1995)4:11<2439:CAPAOF>2.0.ZU;2-3
Abstract
Diffraction-quality crystals of the bifunctional enzyme fructose 6-pho sphate, 2-kinase:fructose 2,6-bisphosphatase from rat testis have been obtained. The crystals were grown in the presence of ATP gamma S, fru ctose 6-phosphate, the detergent n-octylglucoside, and the precipitant polyethylene glycol 4000. The crystals have the symmetry of the trigo nal space group P3(1/2)21 with a = b = 83.0 Angstrom and c = 130.6 Ang strom. Flash-frozen crystals diffract to beyond 2.2 Angstrom, and nati ve data have been collected.