Es. Istvan et al., CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF FRUCTOSE 6-PHOSPHATE, 2-KINASE-FRUCTOSE 2,6-BISPHOSPHATASE, Protein science, 4(11), 1995, pp. 2439-2441
Diffraction-quality crystals of the bifunctional enzyme fructose 6-pho
sphate, 2-kinase:fructose 2,6-bisphosphatase from rat testis have been
obtained. The crystals were grown in the presence of ATP gamma S, fru
ctose 6-phosphate, the detergent n-octylglucoside, and the precipitant
polyethylene glycol 4000. The crystals have the symmetry of the trigo
nal space group P3(1/2)21 with a = b = 83.0 Angstrom and c = 130.6 Ang
strom. Flash-frozen crystals diffract to beyond 2.2 Angstrom, and nati
ve data have been collected.