Dj. Check et al., EFFECT OF PENTOXIFYLLINE ADDED TO FREEZING MEDIA ON SUBSEQUENT POSTTHAW HYPOOSMOTIC SWELLING TEST AND OTHER SEMEN PARAMETERS, Archives of andrology, 35(3), 1995, pp. 161-163
This study evaluated the effects of pentoxifylline (PTX) on post-thaw
semen parameters as well as the hypoosmotic swelling (HOS) test. Fourt
een samples were evaluated for volume, count, motility, % grade A sper
m, and HOS test. Two aliquots were frozen, one in freezing medium and
the other in a 3 mM solution of PTX and freezing medium. Both groups w
ere frozen in liquid nitrogen vapors for 30 min. Thawing was performed
at 37 degrees C for 15 min, followed by a wash with 2 parts 0.5% HSA/
MHTF to 1 part sample. Pellets were resuspended in 0. MHTF and then ev
aluated as described above. In addition, motility was evaluated 2 h po
st-thaw. Following freeze-thaw, the mean motile densities were similar
(17.5 x 10 motile/mL vs. 20.4 x 10(6) motile/mL for PTX and control,
respectively). Two hours post-thaw, the PTX group had a mean sperm mot
ility of 31.3% vs. 37.7% for the control group (p > .05). There were n
o significant differences in % grade A sperm in PTX (43.0%) vs. contro
l (12.0%). Similarly, HOS scores did not improve following cryopreserv
ation (43.0% and 50.6% for PTX and control, respectively). Thus, no im
provement was found by freezing sperm with PTX.