REGULATION OF HUMAN SPERM MOTILITY AND HYPERACTIVATION COMPONENTS BY CALCIUM, CALMODULIN, AND PROTEIN PHOSPHATASES

The role of Ca2+, calmodulin, and protein phosphatases on motility and hyperactivation of non-capacitated, capacitating, and detergent-perme abilized reactivated human sperm was examined. In non-capacitated sper m, W7 inhibited percent motility (%MOT), curvilinear velocity (VCL), a mplitude of lateral head displacement (ALH), and percent hyperactivati on (%HYP) in an extracellular Ca2+ concentration-dependent manner (p < .05). However, in capacitating sperm, inhibition of motility by W7 wa s independent of external Ca2+. Treatment of reactivated sperm with a synthetic calmodulin inhibitor peptide decreased VCL and ALH in a Ca2-dependent manner (p < .05). Ca2+ exhibited a dramatic influence on mo tility within a narrow concentration range (0.7 to 1.0 mu M) in reacti vated sperm. A calmodulin-dependent protein phosphatase (PPZB) was ide ntified by activity assay, immunoblotting, and dephosphorylation of en dogenous phosphoproteins. The sperm enzyme, unlike bovine brain PP2B, was inhibited by 1 mu M okadaic acid (OA) in the presence of Mn2+, sug gesting that the sperm enzyme is unique. In reactivated sperm, inhibit ion of endogenous PP2B-like activity with anti-PP2B antibodies altered ALH, whereas OA altered both VCL and ALH and also inhibited a subset of Ca2+-dependent dephosphorylations of cAMP-dependent phosphoproteins in capacitating sperm. These results suggest (I) an important role fo r calmodulin and PPZB in Ca2+-regulated motility parameters, particula rly ALH, and (2) that modulation of human sperm motility, including hy peractivation by cAMP-dependent phosphorylation, requires calmodulin-d ependent as well as other protein dephosphorylations.