CAN RAPD-PCR BE USED TO DISCRIMINATE BETWEEN THE HYBRIDIZATION GROUPSOF AEROMONAS

Citation
Hj. Oakey et al., CAN RAPD-PCR BE USED TO DISCRIMINATE BETWEEN THE HYBRIDIZATION GROUPSOF AEROMONAS, Medical microbiology letters, 4(7), 1995, pp. 373-381
Citations number
NO
Categorie Soggetti
Microbiology
ISSN journal
10184627
Volume
4
Issue
7
Year of publication
1995
Pages
373 - 381
Database
ISI
SICI code
1018-4627(1995)4:7<373:CRBUTD>2.0.ZU;2-Y
Abstract
We report the preliminary results obtained from an investigation into the suitability of the random amplified polymorphic DNA polymerase cha in reaction to discriminate between the currently recognised DNA hybri disation groups of the genus Aeromonas. The production of a multi-band ed fingerprint was dependent upon the primer used. We show that at lea st one 10-mer primer (OPE-14) will form different fingerprints of mult iple bands for all hybridisation groups, thus suggesting that RAPD-PCR may have potential as a discriminatory tool for aeromonads.