CHARACTERIZATION OF THE LET-653 GENE IN CAENORHABDITIS-ELEGANS

Citation
Sjm. Jones et Dl. Baillie, CHARACTERIZATION OF THE LET-653 GENE IN CAENORHABDITIS-ELEGANS, MGG. Molecular & general genetics, 248(6), 1995, pp. 719-726
Citations number
36
Categorie Soggetti
Genetics & Heredity",Biology
ISSN journal
00268925
Volume
248
Issue
6
Year of publication
1995
Pages
719 - 726
Database
ISI
SICI code
0026-8925(1995)248:6<719:COTLGI>2.0.ZU;2-#
Abstract
A mutation in the let-653 gene of Caenorhabditis elegans results in la rval death. The lethal arrest is concurrent with the appearance of a v acuole anterior to the lower pharyngeal bulb. The position of the vacu ole is consistent with a dysfunction of the secretory/excretory appara tus. Germline transformation rescue experiments were able to position the let-653 gene to two overlapping cosmid subclones. Sequence data ge nerated from both cDNA and genomic DNA subclones indicated that let-65 3 encodes a mucin-like protein. Our characterization suggests that a m ucin-like protein is essential for effective functioning of the secret ory/excretory apparatus within C. elegans.