IMMOBILIZED SOFT-METAL AFFINITY SYSTEM FOR AMINO-ACIDS BASED ON AN 8-HYDROXYQUINOLINE-PD(II) COMPLEX - CHARACTERIZATION USING GLYCINE AS A MODEL

The affinity of glycine for a chromatographic resin modified by covale nt attachment of a complex of 8-hydroxyquinoline with Pd(II) was evalu ated. The loading capacity of the resin for Pd(II) and the correspondi ng reaction equilibria were determined. Experimental, as well as theor etical studies, for the processes of partition and elution of the amin o acid were also undertaken. It was found that for both the partition and elution processes, theoretical predictions based on equilibrium re actions confirmed the experimental data. This study demonstrates that a soft metal, Pd(II), immobilized on a chromatographic resin through i ts interaction with 8-hydroxyquinoline, can be used to adsorb and elut e the amino acid glycine. This could serve as a model for the separati on of amino acids based on immobilized soft-metal affinity chromatogra phy principles.