USE OF NONDENATURING SILVER-STAINED POLYACRYLAMIDE-GEL ANALYSIS OF POLYMERASE CHAIN-REACTION AMPLIFICATION PRODUCTS FOR THE DIFFERENTIAL-DIAGNOSIS OF LEPTOSPIRA-INTERROGANS INFECTION

Authors
OLIVEIRA MAA CABALLERO OL NETO ED KOURY MC ROMANHA AJ CARVALHO J HARTSKEERL RA SIMPSON AJG
Citation
Maa. Oliveira et al., USE OF NONDENATURING SILVER-STAINED POLYACRYLAMIDE-GEL ANALYSIS OF POLYMERASE CHAIN-REACTION AMPLIFICATION PRODUCTS FOR THE DIFFERENTIAL-DIAGNOSIS OF LEPTOSPIRA-INTERROGANS INFECTION, Diagnostic microbiology and infectious disease, 22(4), 1995, pp. 343-348
Citations number
14
Categorie Soggetti
Microbiology,"Infectious Diseases
Journal title
Diagnostic microbiology and infectious disease → ACNP
ISSN journal
07328893
Volume
22
Issue
4
Year of publication
1995
Pages
343 - 348
Database
ISI
SICI code
0732-8893(1995)22:4<343:UONSPA>2.0.ZU;2-Y
Abstract
A 285-bp DNA fragment was amplified using the polymerase chain reactio n from 38 Leptospira serovars of six different genomic species. The fr agments amplified exhibited differential mobilities on nondenaturing p olyacrylamide gels resulting from sequence-dependent conformational al terations. Leptospira interrogans serovars could be distinguished from those of other species on this basis.