SEQUENCING AND LINKAGE ANALYSIS OF A COXIELLA-BURNETII 2.1 KB NOTI FRAGMENT

Authors
WILLEMS H THIELE D KRAUSS H
Citation
H. Willems et al., SEQUENCING AND LINKAGE ANALYSIS OF A COXIELLA-BURNETII 2.1 KB NOTI FRAGMENT, European journal of epidemiology, 11(5), 1995, pp. 559-561
Citations number
NO
Categorie Soggetti
Public, Environmental & Occupation Heath
Journal title
European journal of epidemiology → ACNP
ISSN journal
03932990
Volume
11
Issue
5
Year of publication
1995
Pages
559 - 561
Database
ISI
SICI code
0393-2990(1995)11:5<559:SALAOA>2.0.ZU;2-2
Abstract
Most of the Coxiella burnetii isolates (72 of 80) available in our ins titute share a 2.1 kb NotI fragment. Sequence analysis revealed two in complete open reading frames (ORF) for putative genes hemA and bcR cod ing for glutamyl-tRNA-reductase and bicyclomycin resistance protein, r espectively. Upon completing the ORF for the hemA gene by sequencing t he adjacent NotI/EcoRI fragment a third ORF for the putative prfA gene was determined coding for release factor 1, an enzyme catalysing the last step in protein biosynthesis.