BINDING-SITE FOR BASIC DRUGS ON ALPHA(1)-ACID GLYCOPROTEIN AS REVEALED BY CHEMOMETRIC ANALYSIS OF BIOCHROMATOGRAPHIC DATA

Authors
KALISZAN R NASAL A TUROWSKI M
Citation
R. Kaliszan et al., BINDING-SITE FOR BASIC DRUGS ON ALPHA(1)-ACID GLYCOPROTEIN AS REVEALED BY CHEMOMETRIC ANALYSIS OF BIOCHROMATOGRAPHIC DATA, BMC. Biomedical chromatography, 9(5), 1995, pp. 211-215
Citations number
20
Categorie Soggetti
Chemistry Analytical","Pharmacology & Pharmacy",Biology,"Biochemical Research Methods
Journal title
BMC. Biomedical chromatography → ACNP
ISSN journal
02693879
Volume
9
Issue
5
Year of publication
1995
Pages
211 - 215
Database
ISI
SICI code
0269-3879(1995)9:5<211:BFBDOA>2.0.ZU;2-C
Abstract
Interactions between alpha(1)-acid glycoprotein (AGP) and 52 basic dru gs were quantified by means of high-performance liquid chromatography (HPLC). The HPLC retention parameters were related quantitatively to t he hydrophobicity and molecular modelling parameters, giving rise to t he prediction of relative drug-AGP binding from the chemical structure of a drug. A structural model of one binding site on AGP, common for various classes of drugs, was defined which accounted for the observed and reported differences in binding to AGP. A combination of biochrom atography and chemometrics has been presented as a promising new appro ach in biochemieal/pharmacological studies.