ANTIBACTERIAL PHLOROGLUCINOLS AND FLAVONOIDS FROM HYPERICUM BRASILIENSE

Three known phloroglucinols (japonicine A, uliginosin A and isouligino sin B) and a new phloroglucinol (hyperbrasilol A) have been isolated f rom a petrol extract of the leaves and flowers of Hypericum brasiliens e. Their Structures were established by spectroscopic methods (UV, DCI -MS, H-1 and C-13 NMR, including SINEPT, HMBC, HSQC, DQFCOSY experimen ts). The substitution pattern of hyperbrasilol A was confirmed by X-ra y crystallography. All four phloroglucinols were antibacterial against Bacillus subtilis in a TLC bioautographic assay. The flavonoids, kaem pferol, luteolin, quercetin, quercitrin, isoquercitrin, hyperoside and guaijaverin, were isolated from a methanol extract of the same organs .