Ae. Donascimento et W. Desouza, DOUBLE REPLICAS ALLOW THE OBTENTION OF LONGITUDINALLY FREEZE-FRACTURED TRYPANOSOMA-CRUZI, Biocell, 20(3), 1996, pp. 243-249
The double replica device was used to obtain freeze-fracture replicas
of gently pressed cells, allowing the visualization of a large number
of longitudinally fractured epimastigote and trypomastigote forms of T
rypanosoma cruzi. This technique revealed large areas of the plasma me
mbrane, the region of attachment of the flagellum to the cell body and
the branched mitochondria.