IDENTIFICATION, ISOLATION, AND CLONING OF A BACILLUS-THURINGIENSIS CRYIAC TOXIN-BINDING PROTEIN FROM THE MIDGUT OF THE LEPIDOPTERAN INSECT HELIOTHIS-VIRESCENS

Citation
Ss. Gill et al., IDENTIFICATION, ISOLATION, AND CLONING OF A BACILLUS-THURINGIENSIS CRYIAC TOXIN-BINDING PROTEIN FROM THE MIDGUT OF THE LEPIDOPTERAN INSECT HELIOTHIS-VIRESCENS, The Journal of biological chemistry, 270(45), 1995, pp. 27277-27282
Citations number
56
Categorie Soggetti
Biology
ISSN journal
00219258
Volume
270
Issue
45
Year of publication
1995
Pages
27277 - 27282
Database
ISI
SICI code
0021-9258(1995)270:45<27277:IIACOA>2.0.ZU;2-W
Abstract
Bacillus thuringiensis toxins are insecticidal to a variety of insect species, The selectivity of the toxins produced by these bacteria is d ependent on both the toxin structure and the receptor sites that are p resent in different insect species, One of these toxins, CryIAc, is hi ghly insecticidal to the noctuid pest Heliothis virescens. Using toxin overlay assay, a 120-kDa glycoprotein was identified as a toxin-bindi ng protein. This protein was partially purified, its N-terminal sequen ce was determined, and the full-length cDNA encoding this protein was isolated from a H. virescens midgut library, The B. thuringiensis toxi n-binding protein, BTBP1, has high homology to aminopeptidase N from e ukaryotes and prokaryotes.