SYK TYROSINE KINASE REQUIRED FOR MOUSE VIABILITY AND B-CELL DEVELOPMENT

The Syk cytoplasmic protein-tyrosine kinase has two amino-terminal SH2 domains and a carboxy-terminal catalytic domain(1). Syk, and its clos e relative ZAP-70 (ref. 2), are apparently pivotal in coupling antigen - and Fc-receptors to downstream signalling events(3,4). Syk associate s with activated Fc receptors(5), the T cell receptor complex(6) and t he B-cell antigen-receptor complex (BCR) in immature and mature B lymp hocytes(7). On receptor activation, the tandem SH2 domains of Syk bind dual phosphotyrosine sites in the conserved ITAM motifs of receptor s ignalling chains, such as the immunoglobulin alpha and beta-chains of the BCR, leading to Syk activation(3,4,8). Here we have investigated S yk function in vivo by generating a mouse strain with a targeted mutat ion in the syk gene. Homozygous syk mutants suffered severe haemorrhag ing as embryos and died perinatally, indicating that Syk has a critica l role in maintaining vascular integrity or in wound healing during em bryogenesis. Analysis of syk(-/-) lymphoid cells showed that the syk m utation impaired the differentiation of B-lineage cells, apparently by disrupting signalling from the pre-BCR complex and thereby preventing the clonal expansion, and further maturation, of pre-B cells.