RENAL ABNORMALITIES AND AN ALTERED INFLAMMATORY RESPONSE IN MICE LACKING CYCLOOXYGENASE-II

PROSTAGLANDINS have wide-ranging effects in the body and are thought t o be important mediators of inflammation. Cyclooxygenase (COX) plays a keg regulatory role in prostaglandin synthesis, and occurs in both co nstitutive (COX-1) and inducible (COX-2) isoforms(1,2). COX-1 is thoug ht to provide cytoprotective effects(3), whereas COX-2 is both inducib le and the major isoform of inflammatory cells(4), Reduction of prosta glandin production by inhibition of cyclooxygenases appears to be the main mechanism of action of most non-steroidal anti-inflammatory drugs (NSAIDS)(5), Here we present an animal model of COX-2 deficiency that was generated by gene targeting. Defects in null mice correlating wit h reduced viability included renal alterations, characteristic of rena l dysplasia (100% penetrance), and cardiac fibrosis (50% penetrance). Female Cox-2 -/- mice were infertile, COX-2 deficiency failed to alter inflammatory responses in several standard models, but striking mitig ation of endotoxin-induced hepatocellular cytotoxicity was observed.