HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC D ETERMINATION OF PURINE AND PYRIMIDINE-BASES

Citation
B. Lassalas et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC D ETERMINATION OF PURINE AND PYRIMIDINE-BASES, Annales de zootechnie, 42(2), 1993, pp. 170-171
Citations number
4
Categorie Soggetti
Agriculture Dairy & AnumalScience
Journal title
ISSN journal
0003424X
Volume
42
Issue
2
Year of publication
1993
Pages
170 - 171
Database
ISI
SICI code
0003-424X(1993)42:2<170:HLDEOP>2.0.ZU;2-C
Abstract
In order to estimate the flow of microbial proteins entering the small intestine in ruminants, reversed phase high performance liquid chroma tography (HPLC) was applied to separate purine and pyrimidine bases fo r 12 min. This method was used to analyse duodenal digesta and microbi al pellets isolated from the rumen contents.