PROMOTER ANALYSIS OF THE BLI-7 EAS GENE

Expression of the Neurospora crassa gene bli-7, (identical with eas, a nd ccg-2), is induced by blue light, as well as glucose- or nitrogen-s tarvation. A promoter analysis was performed by an assay that does not involve promoter-reporter constructs but rather the insertion of fore ign DNA into the transcribed sequence. To detect regulatory elements a series of deletions in the upstream region was generated. The inducib ility of the gene, in response to the three inducing conditions mentio ned, is lost by eliminating the region between - 1498 bp and - 1017 bp upstream of the transcription start point. A segment with an apparent ly negative effect was found between - 595 bp and - 429 bp, as well as a stretch of DNA from - 429 bp to - 380 bp which may exert a positive influence after light induction.