DIETARY LIPIDS, PROSTAGLANDIN-E(2) LEVELS, AND TOOTH MOVEMENT IN ALVEOLAR BONE OF RATS

A previous study showed that certain dietary lipids can alter arachido nic acid concentrations in alveolar bone. Because arachidonic acid is a precursor of prostaglandin (PG) E2, which is known to play an import ant role in orthodontic tooth movement, the purpose of the present stu dy was to determine the effect of dietary lipids on PGE, levels and to oth movement. Two groups of male Sprague-Dawley rats (20/group) were f ed nutritionally adequate purified diets containing 10% corn oil (grou p I, rich in n-6 fatty acids) or 9% ethyl ester concentrate of n-3 fat ty acids +1% com oil (group II rich in n-3 fatty acids). After 5 weeks of feeding the diets, orthodontic force of 56 g was applied to the ma xillary incisors to tip them distally. Prior to killing the rats at da y 4 and 8 of orthodontic force application, tooth movement was measure d by computerized image analysis. Pre-maxillae were dissected out free of soft tissue and incisors. The alveolar bone was frozen in liquid n itrogen, pulverized, and lipids were extracted. The concentrations of arachidonic acid and fatty acid composition of total phospholipids wer e measured by gas chromatography. PGE2 levels were measured by enzyme immunoassay. Arachidonic acid and PGE, concentration were significantl y lower (P < 0.001) in alveolar bone of rats in group II than in group I. The tooth movement was also significantly lower (P < 0.02) in grou p II than in group I at both 4 and 8 days. The results suggest that PG E2 levels in alveolar bone and orthodontic tooth movement can be affec ted by the type of dietary fat.