PHOSPHATIDYLSERINE SPECIFIC BINDING-PROTEIN IN RAT-BRAIN - PURIFICATION AND CHARACTERIZATION

A calcium-independent phosphatidylserine specific binding protein dete cted on liposome blotting analysis was purified from rat brain and rev ealed to be identical to myristoylated, alanine-rich C kinase substrat e (MARCKS). MARCKS specifically binds to phosphatidyl-serine but not p hosphatidylcholine. The binding of MARCKS to phosphatidylserine was ab olished on protein kinase C-dependent phosphorylation. Since bacterial ly expressed MARCKS also specifically binds to phosphatidylserine, myr istoylation of the N-terminal glycine seems not to be essential for th e binding of MARCKS to phosphatidylserine. These data suggest that pho sphatidylserine is a membranous target molecule of MARCKS.