Js. Vicente et F. Garciaximenez, EFFECT OF RECIPIENT DOE GENOTYPE ON SURVIVAL RATE AT BIRTH OF FROZEN RABBIT EMBRYOS, Reproduction, nutrition, development, 33(3), 1993, pp. 229-234
The effect of the genotype of 2 selected rabbit strains (White New Zea
land and Synthetic) on the survival rate at birth of frozen embryos wa
s studied. Morulae were obtained 64-66 h post-coitum from 32 multiparo
us does naturally mated with males of the same strain (1 6 does of Syn
thetic strain (SY) and 16 does of the White New Zealand strain (NZ)).
Morphologically normal morulae were slowly frozen in 1.5 M DMSO and st
ored in liquid nitrogen. After thawing, intact embryos from both strai
ns were transferred into the oviducts of 32 recipient does of both str
ains (1 6 SY strain does and 16 NZ does). Significant differences were
observed in pregnancy rate and survival rate at birth between strains
of recipient does. The recipient does of the SY strain showed a highe
r pregnancy rate than those of the NZ strain (87.5% versus 25% respect
ively; P < 0.05). When the survival rate at birth was analyzed in all
recipient does, this was found to be 28 +/- 4% in SY recipient does ve
rsus 8 +/- 4 % in NZ recipient does (P < 0.01). However, when the surv
ival rate was analyzed in pregnant recipient does, no significant diff
erences were found between embryo strains and recipient strains, the a
verage survival rate was almost-equal-to 32%. The major factor in the
differential survival rate between SY and NZ embryos was due to the ge
notype of recipient does.