Ab. Awad et al., EFFECT OF DIETARY PHYTOSTEROLS ON CELL-PROLIFERATION AND PROTEIN-KINASE-C ACTIVITY IN RAT COLONIC MUCOSA, Nutrition and cancer, 27(2), 1997, pp. 210-215
The present study investigated the role of phytosterols in colonic cel
l proliferation and examined the possible role of protein kinase C (PK
C) in this process. A total of 18 male Sprague-Dawley rats weighing 24
0-270 g were fed, for a period of 22 days, one of three experimental d
iets: a control diet, a diet supplemented with 0.2% cholic acid, or a
diet supplemented with 0.2% cholic acid + 2% dietary phytosterols. Two
hours before decapitation, animals were injected with 5'-bromo-2'-deo
xyuridine (BrdU, 50 mg/kg body wt ip). Cell proliferation in the proxi
mal colon was measured using a monoclonal antibody to BrdU. PKC activi
ty in the proximal colonic mucosa was assayed using a myelin basic pro
tein as a substrate. Cell proliferation was significantly increased by
276% with 0.2% cholic acid feeding compared with controls. The presen
ce of 2% phytosterols in the diet abolished the cholic acid-induced hy
perplasia. Cholic acid induced a 31% expansion of the proliferative zo
ne. Only the cytosolic PKC was significantly lower in the phytosterol-
fed group. Neither the total PKC nor the particulate PKC demonstrated
an effect of phytosterols on enzyme activity. fn conclusion, we found
that dietary supplementation with 2% phytosterol has a significant pro
tective effect on enhanced cell proliferation and that this effect is
not mediated through the PKC system.