EPIDERMAL-CELL VIABILITY IN RAT SKIN PRESERVED AT 4-DEGREES-C

To determine the longest storage period for the clinical application o f epidermal cells and a better method of preservation at 4-degrees-C, we stored skin in various fluids and assessed the changes in epidermal cell viability. The time course of changes in the viability of epider mal cells from skin in various preservation fluids was similar to the results obtained with whole skin in previous studies. The best preserv ation fluid was found to be RPMI 1640 medium, and the best method of p reservation was immersion in RPMI 1640 at 4-degrees-C with a medium ch ange every 3 days.