INHIBITION OF AMINOPEPTIDASES BY N-AMINOACYL-O-4-NITROBENZOYL HYDROXAMATES

Authors
DEMUTH HU STOCKEL A SCHIERHORN A FITTKAU S KIRSCHKE H BROMME D
Citation
Hu. Demuth et al., INHIBITION OF AMINOPEPTIDASES BY N-AMINOACYL-O-4-NITROBENZOYL HYDROXAMATES, Biochimica et biophysica acta, 1202(2), 1993, pp. 265-270
Citations number
15
Categorie Soggetti
Biophysics,Biology
Journal title
Biochimica et biophysica acta → ACNP
ISSN journal
00063002
Volume
1202
Issue
2
Year of publication
1993
Pages
265 - 270
Database
ISI
SICI code
0006-3002(1993)1202:2<265:IOABNH>2.0.ZU;2-8
Abstract
10 N-aminoacyl-O-4-nitrobenzoyl hydroxamates were investigated as pote ntial inhibitors of aminopeptidases. While the metal-depending enzymes aminopeptidase M, aminopeptidase P and leucine aminopeptidase were in hibited reversibly by the compounds, the thiol enzyme cathepsin H was inhibited efficently in time-dependent reactions according to its subs trate specificity. N-phenylalanyl-O-4-nitrobenzoyl hydroxamate was sho wn to be most effective, exhibiting a second-order-rate constant of in hibitition of 31 766 M-1 s-1.