RAPID IDENTIFICATION AND CLONING OF BACTERIAL TRANSFERRIN AND LACTOFERRIN RECEPTOR PROTEIN GENES

Authors
OGUNNARIWO JA SCHRYVERS AB
Citation
Ja. Ogunnariwo et Ab. Schryvers, RAPID IDENTIFICATION AND CLONING OF BACTERIAL TRANSFERRIN AND LACTOFERRIN RECEPTOR PROTEIN GENES, Journal of bacteriology, 178(24), 1996, pp. 7326-7328
Citations number
12
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
178
Issue
24
Year of publication
1996
Pages
7326 - 7328
Database
ISI
SICI code
0021-9193(1996)178:24<7326:RIACOB>2.0.ZU;2-F
Abstract
The sequences of genes encoding the transferrin and lactoferrin recept or proteins from several bacterial species were analyzed for areas of identity ire the predicted protein sequences. Degenerate oligonucleoti de primers were designed and tested for their ability to amplify porti ons of the receptor genes, Primer pairs capable of amplifying products of the tbpA/lbpA or tbpB/lbpB genes from all specie possessing these receptors were identified.