ONTOGENY OF GONADAL LUTEINIZING-HORMONE AND FOLLICLE-STIMULATING-HORMONE RECEPTORS IN THE FETAL PIG AND RELATED CHANGES IN GONADOTROPIN ANDTESTOSTERONE SECRETION
B. Goxe et al., ONTOGENY OF GONADAL LUTEINIZING-HORMONE AND FOLLICLE-STIMULATING-HORMONE RECEPTORS IN THE FETAL PIG AND RELATED CHANGES IN GONADOTROPIN ANDTESTOSTERONE SECRETION, Biology of reproduction, 49(3), 1993, pp. 609-616
Fetuses of Large White and Meishan sows were collected at 28, 35, 49,
56, 75, 90, 103, and 113 days of gestation. LH, FSH, and testosterone
concentrations were measured either in amniotic fluid (Days 28-56) or
in arterial umbilical blood (Days 75-113). Gonads were analyzed for th
eir content of LH and FSH receptors and RNA transcripts. Most of these
parameters were similar in the two breeds, except that the mean testo
sterone concentration was higher (p < 0.05) in the plasma of 75-113-da
y Meishan fetuses (309 and 136 pg/ml in males and females, respectivel
y) than in Large White fetuses (152 and 109 pg/ml). Higher testosteron
e concentrations were detected in males than in females, either in amn
iotic fluid (114 vs. 81 pg/ml, p < 0.05) or in plasma (230 vs. 122 pg/
ml, p < 0.01). In contrast, higher gonadotropin concentrations were fo
und in the plasma of females than in that of males (2 vs. 1.6 ng/ml I.
H and 4.2 vs. 1.4 ng/ml FSH, p < 0.05). In the testis, 2-3 pmol/g gona
d of LH and FSH receptors were detected as early as Day 28. This value
increased to 10-13 pmol/g between 35 and 56 days before declining to
3-5 pmol/g after Day 90. In ovaries, LH receptors were detected in the
earlier period (Days 28-56) at 1-3 pmol/g before diminishing to 0.1-0
-4 pmol/g. FSH receptors were higher at Day 28 (1 pmol/g) than at any
subsequent stage (0.1-0.4 pmol/g). The gonadal content of RNA transcri
pts was significantly higher in testes than in ovaries. The changes in
LH and FSH receptors in males may be related to changes in the relati
ve testicular content of Leydig and Sertoli cells. It is likely that t
he low expression of gonadotropin receptors in the ovary is related to
the absence of growing follicles during fetal life, along with a lack
of ovarian steroid negative feedback on pituitary gonadotropin secret
ion. Therefore, in the testis the expression of gonadotropin receptors
after sexual differentiation appeared as constitutive, while in the o
vary some restriction of gonadotropin receptor expression would be exe
rted after 75 days post-coitum (p.c.)