GLYCOGEN-PHOSPHORYLASE ACTIVITY AND IMMUNOREACTIVITY DURING PRENATAL AND POSTNATAL-DEVELOPMENT OF RAT-BRAIN

Catalytic activity and immunoreactivity of glycogen phosphorylase were studied in pre- and postnatal rat brain. The catalytic activity was a ssayed in brain homogenates; immunoreactivity was investigated by immu noblot analysis using a monoclonal anti-bovine brain glycogen phosphor ylase antibody. The cellular localization and intensity of immunoreact ivity were analysed on paraffin-embedded sections utilizing the same m onoclonal antibody. The catalytic activity increased 10-fold from embr yonic day 16 to adult; immunoreactivity became detectable on embryonic day 16 and increased in intensity as the enzyme activity rose to adul t values. The first cellular elements to be stained immunohistochemica lly were ependymal cells lining the ventricles, ependymal cells of the choroid plexus, meningeal cells and a selected population of neurons in the brain stem. The immunoreactivity of plexus cells and meningeal cells was reduced or absent in the adult rat brain. The earliest appea rance of glycogen phosphorylase immunoreactivity in astroglial cells w as seen at postnatal day 9 in the hippocampus. The staining pattern of the adult brain was reached at day 22 post partum. The developmental changes in glycogen deposition and in glycogen phophorylase activity a nd immunoreactivity may indicate a variable physiological role of glyc ogen metabolism for different cell types in the pre- and postnatal per iods.