SPECIFIC N-15 LABELING OF LEUCINE RESIDUES IN HUMAN GROWTH-HORMONE

Authors
CHRISTENSEN T PETERSEN J THEISEN CF BJERREGAARD K KRISTENSEN SM LED JJ
Citation
T. Christensen et al., SPECIFIC N-15 LABELING OF LEUCINE RESIDUES IN HUMAN GROWTH-HORMONE, Acta chemica Scandinavica, 47(10), 1993, pp. 990-993
Citations number
14
Categorie Soggetti
Chemistry,Biology
Journal title
Acta chemica Scandinavica → ACNP
ISSN journal
0904213X
Volume
47
Issue
10
Year of publication
1993
Pages
990 - 993
Database
ISI
SICI code
0904-213X(1993)47:10<990:SNLOLR>2.0.ZU;2-S
Abstract
Biosynthetic human growth hormone (hGH) specifically N-15 labelled in the leucine residues has been obtained by recombinant DNA technology, using N-15-labelled leucine and an E. coli strain that requires leucin e. It is shown that, despite the possibility of minor transaminase act ivity, the labelling on the whole is specific, and that the two-dimens ional H-1-N-15 correlation NMR spectra of hGH can be greatly simplifie d by this methodology.