HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF NEUTRAL OLIGOSACCHARIDES ONA BETA-CYCLODEXTRIN-BONDED PHASE COLUMN

A beta-cyclodextrin bonded phase HPLC column was used for the separati on of neutral oligosaccharides derived from starch, cellulose, pullula n, xylan, inulin, and mannan. Rapid, high-resolution separations resul ted when acetonitrile-water mixtures were used as mobile phases. Reten tion times for oligosaccharides were dependent on both monosaccharide composition and linkage types. Compared to aminoalkyl silica gel phase s, commonly used for carbohydrate analysis, the beta-cyclodextrin bond ed phase appeared to be similar in selectivity and superior in durabil ity. It is anticipated that beta-cyclodextrin bonded phase HPLC column s may be useful alternatives to commonly used aminoalkyl-modified sili ca gels for the separation and analysis of neutral oligosaccharides.