FLAME IONIZATION DETECTOR RESPONSES TO ETHYL-ESTERS OF SAND EEL (AMMODYTES LANCEA) FISH-OIL COMPARED FOR DIFFERENT GAS AND SUPERCRITICAL-FLUID CHROMATOGRAPHIC SYSTEMS

A fish oil fatty acid ethyl ester mixture derived from the sand eel (A mmodytes lancea) has been analysed using various capillary gas chromat ography (GC) and supercritical fluid chromatography (SFC) methods and 39 components including cholesterol have been identified. The results of the analytical SFC and GC experiments are compared showing a good r eproducibility within methods and a fair agreement between methods. Th e advantages and disadvantages of the employment of manual and autosam pler split injection, splitless injection, and cold on-column injectio n in GC as well as the use of polar and non-polar columns in GC and SF C are discussed.