Ga. Dunstan et al., THE EFFECT OF LYOPHILIZATION ON THE SOLVENT-EXTRACTION OF LIPID CLASSES, FATTY-ACIDS AND STEROLS FROM THE OYSTER CRASSOSTREA-GIGAS, Lipids, 28(10), 1993, pp. 937-944
The lipid class compositions of adult Pacific oysters [Crassostrea gig
as (Thunberg)] were examined using latroscan thin-layer chromatography
/flame-ionization detection (TLC/FID), and fatty acid compositions det
ermined by capillary gas chromatography and gas chromatography/mass sp
ectrometry (GC/MS). The fatty acid methyl esters were separated using
argentation TLC and also analyzed as their 4,4-dimethyloxazoline deriv
atives using GC/MS. Major esterified fatty acids in C. gigas were 16:0
, 20:5n-3, and 22.6n-3. C20 and C22 nonmethylene interrupted (NMI) fat
ty acids comprised 4.5 to 5.9% of the total fatty acids. The NMI trien
oic fatty acid 22:3(7,13,16) was also identified. Very little differen
ce was found in the proportions of the various lipid classes, fatty ac
ids or sterols between samples of adult oysters of two different sizes
. However, significant differences in some of the lipid components wer
e evident according to the method of sample preparation used prior to
lipid extraction with solvents. Lyophilization (freeze drying) of samp
les led to a significant reduction in the amounts of triacylglycerols
(TG) extracted by solvents in two separate experiments (7.0 and 52.5%
extracted). Extracts from lyophilized samples had less 16:0, C18 unsat
urated fatty acids, and 24-ethylcholest-5-en-3beta-ol, while C20 and C
22 unsaturated fatty acids comprised a higher proportion of the total
fatty acids. There was no significant change in the amounts of polar l
ipids, total sterols, free fatty acids or hydrocarbons observed in ext
racts from lyophilized samples relative to extracts from nonlyophilize
d samples. Addition of water to the freeze-dried samples prior to lipi
d extraction greatly improved lipid yields and resulted in most of the
TG being extracted.