PRODUCTION OF INTERLEUKIN-5 AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR BY T-CELLS OF PATIENTS WITH BRONCHIAL-ASTHMA IN RESPONSE TO DERMATOPHAGOIDES-FARINAE AND ITS RELATION TO EOSINOPHIL COLONY-STIMULATING FACTOR
T. Kamei et al., PRODUCTION OF INTERLEUKIN-5 AND GRANULOCYTE-MACROPHAGE COLONY-STIMULATING FACTOR BY T-CELLS OF PATIENTS WITH BRONCHIAL-ASTHMA IN RESPONSE TO DERMATOPHAGOIDES-FARINAE AND ITS RELATION TO EOSINOPHIL COLONY-STIMULATING FACTOR, American journal of respiratory cell and molecular biology, 9(4), 1993, pp. 378-385
We investigated the effects of Dermatophagoides farinae (Df) and inter
leukin (IL)-2 on the release of eosinophil colony-stimulating factor (
Eo-CSF) activity from mononuclear cells (MNC) and lymphocytes of patie
nts with bronchial asthma (BA) who were sensitive to Df to clarify its
relationship with IL-5 and granulocyte/macrophage colony-stimulating
factor (GM-CSF). MNC and T cells of patients cultured with IL-2 and Df
released Eo-CSF activity. These Eo-CSF activities were partially inhi
bited by anti-IL-5 and anti-GM-CSF antibodies. In 11 of 15 cases studi
ed, MNC from patients produced GM-CSF in response to IL-2. In four of
15 cases studied, MNC from patients produced GM-CSF in response to Df.
On culture with IL-2 or Df, the releases of IL-5 into the medium by M
NC from individual patients varied. The results indicate that in BA re
sponsiveness of lymphocytes to Df is increased, and suggest that IL-5
and GM-CSF produced by T cells play a role in the induction of eosinop
hilia and the pathogenesis of BA.