Basal and induced levels of recA expression in wild-type and isogenic
derivatives of Escherichia coli carrying various rec mutations were me
asured using a low-copy number recA(po)-lacZ fusion, pKLC3.2. Basal re
cA expression in wild-type and isogenic derivatives containing single
rec- mutations, as well as in the recBCsbcA strain and isogenic recA,
recF and recJ derivatives, ranged from 1000 to 3900 units. In the recB
CsbcBC strain and isogenic recL and recN derivatives basal recA expres
sions were 3- to 5-fold higher than that of wild-type cells and were i
nducible by mitomycin C. Except for the recA and lexA3(lnd-) mutants,
recA expression was induced by mitomycin C in wild-type cells and its
isogenic recB, recD, recF, recG, recJ, recL, recN, recO and ruv deriva
tives. RecF was required for induction of recA expression by mitomycin
C, but not by naladixic acid in the recBCsbcA and recBCsbcBC genetic
backgrounds. In wild-type cells, induction of recA expression by nalad
ixic acid required the recBC, but not the recD function of the RecBCD
enzyme. This requirement is suppressed by either an additional sbcA or
sbcC mutation, but not by an sbcB mutation.