INFLUENCE OF GLUCOSE ON THE DEOXYGLUCOSE METABOLISM IN SACCHAROMYCES-CEREVISIAE - DETECTION AND IDENTIFICATION OF DEOXYGLUCOSE AND TREHALOSE DERIVATIVES BY H-1-NMR AND C-13-NMR SPECTROSCOPY

The metabolism of 2-deoxy-D-glucose (DG) in glucose grown repressed Sa ccharomyces cerevisiae cells was studied in the absence and presence o f glucose (Glc) at 30-degrees-C in a standard pyrophosphate medium con taining 4.5 x 10(7) cells/ml. H-1-and C-13-NMR spectroscopy were succe ssfully used to distinguish and identify several derivatives of DG and trehalose. Using [1-C-13]DG, alpha- and beta-DG, alpha- and beta-DG6P , dideoxy-trehalose (DG-DG) and deoxy-trehalose (DG-Glc) can be simult aneously observed in the intracellular medium. The [DG6P]/[DG] ratio i s about 5-6. The results seem to indicate the existence of an equilibr ium between DG and DG6P, which limits the production of DG6P in cells. Glucose was found to exert a great influence on the metabolism of DG. It favours the formation of DG-DG and DG-Glc.